CYP11B2 Polyclonal Antibody

Catalogue Numbers: BS60673-50, BS60673-100

Sizes: 50µl, 100µl

Alternative Name: Cytochrome P450 11B2, mitochondrial; Aldosterone synthase; ALDOS; Aldosterone-synthesizing enzyme; CYPXIB2; Cytochrome P-450Aldo; Cytochrome P-450C18; Steroid 18-hydroxylase; CYP11B2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P19099

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The steroid 11beta-hydroxylase gene, also designated Cyp11b-1, is a marker for the functional differentiation of cells in the zonae fasciculata reticularis. The deduced protein CYP11B1 consists of 466 amino acids containing a secretory signal, epidermal growth factor-like repeats, and a proteolytically inactive cathepsin B-related sequence. A related protein, human aldosterone synthase (CYP11B2), is involved in substrate recognition and conversion, with a functionally significant residue 112 in the N-terminal region of human CYP11B2. The inherited disorder glucocorticoid-remediable aldosteronism iscaused by a chimeric gene duplication between the CYP11B1 and CYP11B2 genes. This disorder is characterized by hyperaldosteronism and high levels of 18-hydroxycortisol and 18-oxocortisol, which are under ACTH control.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CYP11B2 polyclonal antibody detects endogenous levels of CYP11B2 protein.

Bioworld Molecular Weight: ~ 58 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CYP11B2 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human CYP11B2

Conjugate: Unconjugated

Modification: Unmodified