Cytokeratin 8/18 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90389-50, BS90389-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05783(Human) P05787(Human) P05784(Mouse) P11679(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, IP, , FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. They have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 8 expression is seen in epithelium and epithelium-derived tumors. The Cytokeratin 8 and 18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8 and 18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8 and 18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis. Cytokeratin 8 expression correlates with malignancy in leukoplakia and carcinomas of the head and neck; it is expressed in all non-small-cell lung cancers. Cytokeratin 8 has been shown to possess extracellular epitopes on tumor cells, which may represent valuable targets for therapy.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cytokeratin 8/18 polyclonal antibody detects endogenous levels of Cytokeratin 8/18 protein.

Bioworld Molecular Weight: 53 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cytokeratin 8+18 on different lysates using anti-Cytokeratin 8+18 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: A431, ICC staining Cytokeratin 8+18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CK-8, K8, KRT8, CYK8, CK-18, K18, KRT18, CYK18, CARD2, Cell proliferation inducing gene 46 protein, Cell proliferation inducing protein 46, CK 8, CK-8, CK18, CK8, CYK18, CYK8, Cytokeratin 18, Cytokeratin 8, Cytokeratin-8, K18, K2C8, K2C8, K8, Keratin 18, Keratin 8, Keratin, Keratin type I cytoskeletal 18, keratin type II cytoskeletal 8, Keratin-8, KO, KRT18, KRT8, type II cytoskeletal 8, Type-II keratin Kb8,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified