Human ProIAPP (Proislet Amyloid Polypeptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2664-48, ELK2664-96, ELK2664-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Pro-IAPP; Pro-Amylin; Pro-Islet Amyloid Polypeptide

Assay Type: Competitive Inhibition

Sensitivity: 8.9 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Apoptosis; Infection immunity; Hepatology; Gastroenterology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ProIAPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ProIAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ProIAPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only