APOC4 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS60500-50, BS60500-100

Sizes: 50µl, 100µl

Swiss-Prot: P55056

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: Apolipoproteins are protein components of plasma lipoproteins (1). The apolipoprotein C gene family encodes four homologous proteins designated apoC-I to -IV, which specifically modulate the metabolism of triglyceride-rich lipoproteins (2). The human apoC-I gene maps to chromosome 19q13.2 and is expressed primarily in the liver where it is activated when monocytes differentiate into macrophages (3,4). The human apoC-II gene maps to chromosome 19q13.2 and encodes a 79 amino acid single chain protein that is a necessary cofactor for the activation of lipoprotein lipase, the enzyme that hydrolyzes triglycerides in plasma and transfers the fatty acids to tissues (5–7). The human apoC-III gene maps to chromosome 11q23 and encodes a protein that may delay catabolism of triglyceride-rich particles by inhibiting lipoprotein lipase and hepatic lipase (8). The human apoC-IV gene maps to chromosome 19q13.2 and encodes a 127 amino acid protein that is primarily expressed in the liver (9,10).

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 105% (by SDS-PAGE).

Specificity: APOC4 polyclonal antibody detects endogenous levels of APOC4 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of APOC4 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: H9C12 whole cell lysate, Immunohistochemistry (IHC) analyzes of APOC4 pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing secreted staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 15 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -30°C long term. Avoid freeze-thaw cycles.

Alternative Name: Apolipoprotein C-IV; Apo-CIV; ApoC-IV; Apolipoprotein C4; APOC4

Immunogen: A synthetic peptide corresponding to residues in Human APOC4.

Conjugate: Unconjugated

Modification: Unmodified