Parvalbumin (PVALB) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79837-50, BS79837-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P20472

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 15KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Parvalbumin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s., Western blot analysis of extracts of various cell lines, using Parvalbumin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunohistochemistry of paraffin-embedded human esophageal using Parvalbumin) Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: D22S749; Parvalbumin; PVALB; parvalbumin

Immunogen: Recombinant fusion protein of human Parvalbumin (Parvalbumin (PVALB))(NP_002845.1).

Conjugate: Unconjugated

Modification: Unmodified