Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)

Sizes: 96T, 500 Assays

Catalogue Numbers: E-BC-K261-M-96, E-BC-K261-M-500

Citations, Manuals and MSDS Available upon request.

Target Synonym: TG

Research Areas: Metabolic Diseases;Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: serum, plasma and tissue

Assay Time: 30 min

Detection Type: Colorimetric method

Sensitivity: 0.14 mmol/L

Detection Range: 0.14-10 mmol/L

Test Principle: Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK) . Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO) . In the presence of 4-aminoantipyrine and phenol, hydrogen peroxide is catalyzed by peroxidase (POD) to produce quinones which is proportional to the content of TG.

Inter CV: 9.2

Intra CV: 4.1

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag