C4A/C4B Polyclonal Antibody

Catalogue Numbers: BS60394-50, BS60394-100

Sizes: 50µl, 100µl

Alternative Name: Complement C4-A; Acidic complement C4
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; Complement C4 beta chain; Complement C4-A alpha chain; C4a anaphylatoxin; C4b-A; C4d-A; Complement C4 gamma chain; C4A; CO4, CPAMD2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P0C0L4/P0C0L5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The complementcomponent proteins C3, C4 and C5 are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactionsspecificto cells of hematopoieticlineage. These proteins belong to theα2-macroglobulin family, but retain distinctive featuresincluding an anaphylatoxin domain and a netrin (NTR) domain. The human C4 gene is polymorphic at two loci, C4A and C4B,mapping to chromosome 6p21.32. C4A
expressesthe Rodgers(Rg) blood group Ag, while C4B expressesthe Chido(Ch) blood group Ag. C4 is expressed as a precursor that iscleaved into α, β and γ chains, all ofwhich are non-identicalcleavage products. Theαchain of C4may be cleaved to produce an acidicisotype, C4a,which reactswith amino groups, and a basicisotype, C4b,which reactswith hydroxyl groups. Deficiency in the C4 gene is associatedwith autoimmune or immune complex disorders, such assystemiclupus erythematosus.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: C4A/C4B polyclonal antibody detects endogenous levels of C4A and C4B protein.

Bioworld Molecular Weight: ~ 193 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of C4A/C4B polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human C4A.

Conjugate: Unconjugated

Modification: Unmodified