BRCA2 (N60) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2864-50, BS2864-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51587

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: BRCA 2 is localized to chromosome 13q12 - q13. Loss of heterozygosity of 13q is observed in 25% of sporadic breast tumors, which indicates that BRCA 2 might be a tumor suppressor gene. BRCA 2 confers only a low ovarian cancer risk. Reportedly, in MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA 2 RNA lowers in G0 and early G1 phases then gets up-regulated at the G1 / S phase junction.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of BRCA2 (N60) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 384 kDa

Specificity: BRCA2 (N60) polyclonal antibody detects endogenous levels of BRCA2 protein.

Alternative Name: Fanconi anemia group D1 protein; Breast cancer type 2 susceptibility protein; BRCA2; FACD; FANCD1

Immunogen: Synthetic peptide, corresponding to amino acids 60-100 of Human BRCA2.

Conjugate: Unconjugated

Modification: Unmodified