Histone H2B (mono methyl R79) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS94094-50, BS94094-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60814(Human) P06899(Human) P23527(Human) P33778(Human) Q64524(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Histone H2B (mono methyl R79) polyclonal antibody detects endogenous levels of Histone H2B protein only when mono-methylated at R79.

Bioworld Molecular Weight: 14 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Histone H2B(mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Histone H2B(mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Hist2h2be, H2B 1A, H2B, H2B histone family, H2B2f, H2B2F, H2Ba, H2Bf, HIST2H2BF, histone H2B, histone H2B type 1, Histone H2B type 2-F, MGC131639,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Methylated