BUB1 rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES7356-50, ES7356-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],

Alternate Name: BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A

Source: Rabbit

Applications: WB; ELISA

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Reactivity: Human; Rat; Mouse;

Immunogen: The antiserum was produced against synthesized peptide derived from human BUB1. AA range:781-830

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 120kD

Human Gene ID: 699

Human SWISS Prot NO: O43683

Subcellular Location: Nucleus. Chromosome, centromere, kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, KNL1 and INCENP are required for kinetochore localization (By similarity).

Research Use Only