NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups.

Product information

CAS Number: 10199-89-0

Molecular Weight: 199.55

Formula: C6H2ClN3O3

Chemical Name: 4-chloro-7-nitro-2,1,3-benzoxadiazole

Smiles: [O-][N+](=O)C1=CC=C(Cl)C2=NON=C12

InChiKey: IGHBXJSNZCFXNK-UHFFFAOYSA-N

InChi: InChI=1S/C6H2ClN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : ≥ 100 mg/mL (501.13 mM). H2O : 0.67 mg/mL (3.36 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

NBD-Cl (NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides.

Products are for research use only. Not for human use.