14-3-3 θ (W228) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1790-50, BS1790-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P27348

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Protein interactions with 14-3-3 show distinct preferences for it’s different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 tau/theta is primarily expressed in T-cells although it occurs at lower levels in the brain, heart, pancreas placenta and kidneys. It plays a role in cell cycle progression via interaction with p27(Kip1). Along with COPI, the theta isoform binds the GB1 RSR sequence involved in protein trafficking. The isoform forms a complex with Hsp60 and cellular prion protein which may be involved in prion diseases. Elevated levels of 14-3-3 tau/theta are observed in amyotrophic lateral sclerosis. 14-3-3 tau/theta also binds BCR/Abl, bacterial effector protein Tir, yes-associated protein and the FSH receptor.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: 14-3-3 θ (W228) polyclonal antibody detects endogenous levels of 14-3-3 protein theta/tau.

Bioworld Molecular Weight: ~ 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of 14-3-3 θ (W228) polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: C6 whole cell lysate (40ug)
Lane 3: NIH-3T3 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of 14-3-3 θ (W228) pAb in paraffin-embedded human breast carcinoma tissue at 1:50. showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of 14-3-3 θ (W228) pAb in paraffin-embedded human kidney carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., IF image of BS1790 stained A375 cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody 14-3-3 θ (W228) pAb #BS1790(1:100) at 10µg/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Mouse IgG (H+L) FITC#BS10950 used at a 1/400 dilution for 2h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).

Alternative Name: 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; YWHAQ; 14-3-3 θ; 14-3-3 τ; 1433T

Immunogen: Synthetic peptide, corresponding to amino acids 200-240 of Human 14-3-3 θ.

Conjugate: Unconjugated

Modification: Unmodified