DGK-δ (S66) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2078-50, BS2078-100

Sizes: 50µl, 100µl

Swiss-Prot: Q16760

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Diacylglycerol kinases (DGKs) phosphorylate diacylglycerol (DAG) to produce phosphatidic acid. DAG and phosphatidic acid are lipids that act as second messengers in signaling cascades. DGK-α influences cell activation and secretion of lethal exosomes, which in turn control cell death. DGK-β is abundant in restricted brain regions such as the caudate putamen and olfactory tubercle. DGK-γ encodes full-length and truncated transcripts that are present in a range of human tissues, with greatest expression observed in retina. DGK-δ is most abundant in skeletal muscle. DGK-ε shows specificity for arachidonylcontaining diacylglycerol and is expressed predominantly in testis. DGK-θ is most abundant in the cerebellum and hippocampus. DGK-ι is present in brain and retina as a predominant transcript of more than 12 kb, including a long 3-prime untranslated region, with additional low abundance transcripts of 9.5 and 7.5 kb. DGK-η is closely related to DGK-δ. DGK-ζ is most abundant in brain and muscle. DGKs have structural motifs that play regulatory roles, and these motifs form the basis for dividing the DGKs into five subtypes.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: DGK-δ (S66) polyclonal antibody detects endogenous levels of DGK-δ protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DGK-δ (S66) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: Hela whole cell lysate (40ug)
Lane 3: A2780 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: 3T3-L1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of DGK-δ (S66) pAb in paraffin-embedded human Rectum carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 135 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Diacylglycerol kinase delta; DAG kinase delta; 130 kDa diacylglycerol kinase; Diglyceride kinase delta; DGK-delta; DGKD; KIAA0145; DGKδ; DGK δ

Immunogen: Synthetic peptide, corresponding to amino acids 34-88 of Human DGK-δ.

Conjugate: Unconjugated

Modification: Unmodified