Rat NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8211-48, ELK8211-96, ELK8211-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP (1-32); BNP (5-29); BNP-32; Gamma-brain natriuretic peptide; nppb

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates,cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Endocrinology; Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only