ZM223 hydrochloride is an orally active, potent non-covalent NEDD8 activating enzyme (NAE) inhibitor with excellent anticancer activity.

Product information

CAS Number: 2438679-27-5

Molecular Weight: 538.99

Formula: C23H18ClF3N4O2S2

Chemical Name: N-(6-{2-[(4-aminophenyl)sulfanyl]acetamido}-1,3-benzothiazol-2-yl)-4-(trifluoromethyl)benzamide hydrochloride

Smiles: Cl.NC1C=CC(=CC=1)SCC(=O)NC1=CC=C2N=C(NC(=O)C3C=CC(=CC=3)C(F)(F)F)SC2=C1

InChiKey: DXCZCDSLNIYJQF-UHFFFAOYSA-N

InChi: InChI=1S/C23H17F3N4O2S2.ClH/c24-23(25,26)14-3-1-13(2-4-14)21(32)30-22-29-18-10-7-16(11-19(18)34-22)28-20(31)12-33-17-8-5-15(27)6-9-17;/h1-11H,12,27H2,(H,28,31)(H,29,30,32);1H

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 150 mg/mL (278.30 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

ZM223 hydrochloride (0.1-1 μM; 4 hours) inhibits both HCT-116 and U-2OS cancer cells with IC50s of 100 and 122 nM, respectively. ZM223 hydrochloride (0.1-1 μM; 4 hours) causes a dose-response decrease in the level of NEDD8 and accumulation of the UBC12 protein, indicating the decrease of the subsequent NEDD8-UBC12 complex.

Products are for research use only. Not for human use.