Acrp30 (R55) Polyclonal Antibody

Catalogue Numbers: BS2833-50, BS2833-100

Sizes: 50µl, 100µl

Alternative Name: Adiponectin; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte; C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; ACDC; ACRP30; APM1; GBP28; ADIPOQ

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15848

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Adiponectin, also termed AdipoQ, Acrp30, apM1 and GBP28, is an adipokine expressed exclusively in brown and white adipocytes. It is secreted into the blood and exists in three major forms: a low molecular weight trimer, a medium molecular weight hexamer and a high molecular weight multimer. Adiponectin levels are decreased in obese and insulin-resistant mice and humans, suggesting that this adipokine is critical to maintain insulin sensitivity. Adiponectin stimulates the phosphorylation of AMPKα at Thr172 and activates AMPK in skeletal muscle. It also stimulates glucose uptake in myocytes. The block of AMPK activation by a dominant-negative AMPKα2 isoform inhibits the effect of adiponectin on glucose uptake, indicating that adiponectin stimulates glucose uptake and increases insulin sensitivity through its action on AMPK. Adiponectin mutants that are not able to form oligomers larger than trimers have no effect on the AMPK pathway. Mutations that render adiponectin unable to form high molecular weight multimers are associated with human diabetes, indicating the importance of multimerization for adiponectin activity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Acrp30 (R55) polyclonal antibody detects endogenous levels of Acrp30 protein.

Bioworld Molecular Weight: ~ 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Acrp30 (R55) polyclonal antibody at 1:1000 dilution
Lane 1: MCF-7 whole cell lysate(40ug)
Lane 2: The testis tissue lysate of Mouse(40ug)
Lane 3: The liver tissue lysate of Rat(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 22-76 of Human Acrp30.

Conjugate: Unconjugated

Modification: Unmodified