SAMHD1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91202-50, BS91202-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y3Z3(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: SAM domain and HD domain-containing protein 1 is a protein that in humans is encoded by the SAMHD1 gene. SAMHD1 is a cellular enzyme, responsible for blocking replication of HIV in dendritic cells, macrophages and monocytes. It is an enzyme that exhibits phosphohydrolase activity, converting deoxynucleoside triphosphates (dNTPs) to inorganic phosphate (iPPP) and a 2'-deoxynucleoside (i.e. deoxynucleosides without a phosphate group). In doing so, SAMHD1 depletes the pool of dNTPs available to a reverse transcriptase for viral cDNA synthesis and thus prevents viral replication. SAMHD1 has also shown nuclease activity. Although a ribonuclease activity was described to be required for HIV-1 restriction, recent data confirmed that SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: SAMHD1 polyclonal antibody detects endogenous levels of SAMHD1 protein.

Bioworld Molecular Weight: 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of SAMHD1 on THP-1 cell lysate using anti-SAMHD1 antibody at 1/1,000 dilution., ICC staining SAMHD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: dNTPase, DCIP, MOP-5, SAMHD1, MOP5, CHBL2, DCIP, Dendritic cell derived IFNG induced protein, Dendritic cell-derived IFNG-induced protein, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, dNTPase, HD domain containing 1, HDDC1, Mg11, Monocyte protein 5, MOP 5, MOP-5, MOP5, OTTHUMP00000030889, SAM domain and HD domain 1, SAM domain and HD domain containing protein 1, SAM domain and HD domain-containing protein 1, SAMH1, Samhd1, SBBI88,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified