RAF1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-60020-60, E-AB-60020-120, E-AB-60020-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: RAF1

Target Synonym: CMD1NN; CRAF; NS5; Raf-1; c-Raf; RAF1

Research Areas: Cancer, Cell Biology, Metabolism, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P04049

Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human RAF1 (NP_002871.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200

Calculated MW: 73kDa/75kDa

Observed MW: 73kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only