GLRB Polyclonal Antibody Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Sizes: 50µl, 100µl Catalogue Numbers: BS65856-50, BS65856-100 Swiss-Prot: P48167 Host: Rabbit GLRB Polyclonal Antibody-BS65856 Storage and Stability: Store at 4°C short term. Aliquot and store at -29°C long term. Avoid freeze-thaw cycles. Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Applications: IHC-P All Applications: IHC-P=1:100-500 Background: GLRB (Glycine receptor beta) is a neurotransmitter-gated ion channel concentrated within the spinal cord and brainstem. Expression is also observed in several upper brain regions including the cortex, cerebellum, hippocampus and amygdala. Binding of glycine to GLRB increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing), controlling spinal reflexes and locomotor behavior. Purification and Purity: affinity purified by Protein A Specificity: GLRB Polyclonal Antibody detects endogenous levels of GLRB protein. Bioworld Molecular Weight: ~54kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLRB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (rat brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLRB) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C Alternative Name: Glycine receptor 58 kDa subunit; Glycine receptor beta; Glycine receptor subunit beta; Glycine receptor, beta subunit; GLRB_HUMAN. Immunogen: KLH conjugated synthetic peptide derived from human GLRB:51-150/497 Modification: Unmodified Conjugate: Unconjugated