Adducin α/β (phospho-S726/713) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS4005-50, BS4005-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35611/P35612

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-Adducin α/β (S726/713) polyclonal antibody detects endogenous levels of Adducin α protein only when phosphorylated at Ser726 and Adducin β protein only when phosphorylated at Ser713.

Bioworld Molecular Weight: ~ 81 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-Adducin α/β (S726/713) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate treated with PMA
Lane 2: SP2/0 cell lysate treated with PMA
Lane 3: PC12 cell lysate treated with PMA, Immunohistochemistry (IHC) analyzes of Adducin α/β (phospho-S726/713) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Adducin α/β (phospho-S726/713) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50, showing cytoplasm and membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Alpha-adducin; Erythrocyte adducin subunit alpha; ADD1; ADDA; α-Adducin; Adducin1; Adducin-1; α Adducin; Adducin-α; Beta-adducin; Erythrocyte adducin subunit beta; ADD2; ADDB; β-Adducin; Adducin1; Adducin-1; β Adducin; Adducin β; Adducin-β

Immunogen: Synthetic phosphopeptide derived from human Adducin α around the phosphorylation site of Serine 726.

Conjugate: Unconjugated

Modification: Phosphorylation