ATP5H (D146) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3812-50, BS3812-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75947

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:100

Background: ATP5H (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit δ), also known as ATPQ, is a 161 amino acid protein that belongs to the ATPase δ subunit family. F-type ATPases, such as ATP5H, consist of two linked components: CF1, a soluble catalytic core that consists of five different subunits (α, β, γ, δ and ε), and CF0, a membrane proton channel that contains nine subunits (α, β, χ, δ, ε, φ, γ, F6 and 8). ATP5H encodes the δ subunit of the F0 complex. ATP5H produces ATP from ADP in the presence of a proton gradient across the membrane, which is generated by electron transport complexes of the respiratory chain. Localizing to mitochondrial inner membrane,

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ATP5H (D146) polyclonal antibody detects endogenous levels of ATP5H protein.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ATP5H (D146) pAb at 1:500 dilution
Lane 1: Hela whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: H1792 whole cell lysate (40ug)
Lane 4: C6 whole cell lysate (40ug)
Lane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of ATP5H (D146) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of HEK293T cell lysate using ATP5H (D146) polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using ATP5H (D146) #BS3812. “↑”（supernatant）;“↓(deposition）

Alternative Name: ATP synthase subunit d, mitochondrial; ATPase subunit d; ATP5H; ORF Names: My032

Immunogen: Synthetic peptide, corresponding to amino acids 121-170 of Human ATP5H.

Conjugate: Unconjugated

Modification: Unmodified