Beta-tubulin Monoclonal Antibody

Catalogue Numbers: MB65889-50, MB65889-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Host: Mouse

Reactivity: Zebrafish

Applications: WB

All Applications: WB (1/2000 - 1/5000)

Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977).
Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Beta-tubulin protein.

Extra Notes: Western blot analysis of Beta-tubulin expression in zebrafish skeletal muscle (A) whole cell lysates.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of zebrafish Beta-tubulin. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified