Cdc7 (F35) Polyclonal Antibody

Catalogue Numbers: BS2447-50, BS2447-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00311

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: DNA replication in eukaryotic cells is dependent on the phosphorylation of the pre-replicative complex (preRC) at the origin of replication. Two complexes of proteins mediate this event, the cyclin dependent kinase (CDK) complex, and the Cdc7 kinase-ASK complex. Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. The activity of Cdc7 kinase oscillates during cell cycle. The major targets of Cdc7 kinase are proteins that belong to the MCM complex (mini chromosome maintenance proteins). Cdc7 kinase was also found to be important in meiosis, checkpoint responses, maintenance of chromosome structure, and repair.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cdc7 (F35) polyclonal antibody detects endogenous levels of Cdc7 protein.

Extra Notes: Western blot (WB) analysis of Cdc7 (F35) polyclonal antibody at 1:500 dilution
Lane1:Hela cell lysate
Lane2:sp2/0 cell lysate
Lane3:H9C2 cell lysate

Bioworld Molecular Weight: ~ 64 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Cell division cycle 7-related protein kinase; CDC7-related kinase; HsCdc7; huCdc7; CDC7; CDC7L1

Immunogen: Synthetic peptide, corresponding to amino acids 11-50 of Human Cdc7.

Conjugate: Unconjugated

Modification: Unmodified