Recombinant Human/Mouse FGF-8b

Size: 10ug

Catalogue Number: EPT144-10

Citations, Manuals and MSDS Available upon request.

Background: Fibroblast growth factor 8 (FGF­8) is a member of the fibroblast growth factor family. It is discovered as a growth factor essential for the androgen-­dependent growth of mouse mammary carcinoma cells. Mouse FGF­8b shares 100% aa identity with human FGF­8b. FGF­8 is widely expressed during embryogenesis, and mediates epithelial­-mesenchymal transitions. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. It is required for normal brain, eye, ear, limb development during embryogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.

Alternate Names: Fibroblast growth factor 8; Androgen-induced growth factor; Heparin-binding growth factor 8; AIGF; HBGF-8; FGF-8B

Description: Recombinant Human/Mouse Fibroblast Growth Factor 8B is produced by our E.coli expression system and the target gene encoding Gln23-Arg215 is expressed.

Accession: P55075-3/P37237-2

Molecular Weight: 22.5 KDa

Apparent Molecular Weight: 23 KDa, reducing conditions

Storage Condition: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.

Biological Activity: Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 21.87 ng/ml.

Redissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Research Use Only