TCTN1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS76032-50, BS76032-100

Swiss-Prot: Q2MV58

Host: Rabbit

TCTN1 Polyclonal Antibody-BS76032

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of a family of secreted and transmembrane proteins. The orthologous gene in mouse functions downstream of smoothened and rab23 to modulate hedgehog signal transduction. This protein is a component of the tectonic-like complex, which forms a barrier between the ciliary axoneme and the basal body. A mutation in this gene was found in a family with Joubert syndrome-13. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 63kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using TCTN1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunofluorescence analysis of U2OS cells using TCTN1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: TCTN1; JBTS13; TECT1

Immunogen: Recombinant fusion protein of human TCTN1(NP_001076006.1).

Modification: Unmodified

Conjugate: Unconjugated