Mouse RPS6KA3 (Ribosomal Protein S6 Kinase Alpha 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0489-48, ELK0489-96, ELK0489-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 90 kDa ribosomal protein S6 kinase 3 (p90-RSK 3; p90RSK3) MAP kinase-activated protein kinase 1b (MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b) Ribosomal S6 kinase 2 (RSK-2) pp90RSK2

Assay Type: Sandwich

Sensitivity: 6.94 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RPS6KA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RPS6KA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RPS6KA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RPS6KA3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only