HASPIN Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77940-50, BS77940-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8TF76

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:1000 - 1:2000

Background: Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using HASPIN antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 100KDa

Alternative Name: GSG2

Immunogen: Recombinant fusion protein of human HASPIN(NP_114171.2).

Conjugate: Unconjugated

Modification: Unmodified