CABYR Polyclonal Antibody

Catalogue Numbers: BS60128-50, BS60128-100

Sizes: 50µl, 100µl

Alternative Name: Calcium-binding tyrosine phosphorylation-regulated protein; Calcium-binding protein 86; Cancer/testis antigen 88; CT88; Fibrousheathin II; Fibrousheathin-2; FSP-2; Testis-specific calcium-binding protein CBP86; CBP86; FSP2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75952

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Spermatozoa gain fertilization capacitation and hyperactivation after residing in the uterus or oviduct. Calcium, cAMP and protein tyrosine phosphorylation are necessary for the molecular mechanisms that allow for this transformation. CABYR (Calcium-binding tyrosine phosphorylation-regulated protein), also known as Fibrousheathin-2 or Cancer/testis antigen 88, is a 493 amino acid protein that is expressed in sperm flagella and exhibits increased tyrosine phosphorylation during capacitation. There are six named isoforms of CABYR that are produced as a result of alternative slicing events. Specifically, isoform 1 is expressed in the testis, while isoform 3 and isoform 5 are expressed in pancreas, brain and various brain tumors. Isoforms 1, 2 and 6 most likely bind calcium, whereas isoforms 3, 4 and 5 probably do not bind calcium.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CABYR polyclonal antibody detects endogenous levels of CABYR protein.

Bioworld Molecular Weight: ~ 30, 53 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CABYR polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human CABYR.

Conjugate: Unconjugated

Modification: Unmodified