GCS-β-1 (V21) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1158-50, BS1158-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02153

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:50~1:200

Background: Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide and a membrane-bound receptor form (GC), which are peptide hormone receptors. The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain. It is expressed as two differentially glycosylated forms, a 130 kDa precursor form present in the endoplasmic reticulum and a 145 kDa form present on the plasma membrane. Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP. GC-C acts as the receptor for heatstable enterotoxins, small peptides secreted by some pathogenic strains of E. coli that cause severe secretory diarrhea. GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GCS-β-1 (V21) polyclonal antibody detects endogenous levels of Guanylate Cyclase β1/3 protein.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GCS-β-1 (V21) polyclonal antibody at 1:500 dilution
Lane 1: THP-1 whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of GCS-β-1 (V21) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of the Brain tissue lysate of Mouse using GCS-β-1 (V21) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using GCS-β-1 (V21) pAb #BS1158. “↑”(supernatant); “↓”(deposition)

Alternative Name: Guanylate cyclase soluble subunit beta-1; GCS-beta-1; Guanylate cyclase soluble subunit beta-3; GCS-beta-3; Soluble guanylate cyclase small subunit; GUCY1B3; GUC1B3; GUCSB3; GUCY1B1; GCS-β1; GCS β1; GCSβ1; GCS-β3; GCS β3; GCSβ3; GCS-β-3

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human GCS-β-1.

Conjugate: Unconjugated

Modification: Unmodified