RARα Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91156-50, BS91156-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10276(Human) P11416(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, FC

All Applications: WB:1:1,000
FC:1:50-1:100

Background: Retinoids (RA) are metabolites of vitamin A (retinol) that are important signaling molecules during vertebrate development and tissue differentiation. RAs activate the retinoic acid receptor (RAR) and retinoid X receptor (RXR) nuclear transcription factor families. Most retinoid forms activate RAR family members, whereas RXR family members are activated by 9-cis-RA only. RAR family members, which include RARα, RARβ and RARγ, have a high affinity for all transretinoic acids and belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. RAR isoforms are expressed in distinct patterns throughout development and in the mature organism. The human RARα gene maps to chromosome 17 and is implicated in the chromosomal translocation associated with acute promyelocytic leukemia (APL-M3). Specifically, the RARα gene is fused with the promyelocytic leukemia (PML) gene, which encodes the fusion protein PML/RARα. The PML/RARα fusion protein inhibits PML-dependent apoptotic pathways and halts myeloid differentiation at the promyelocytic stage.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Flow cytometric analysis of MCF-7 cells with RAR-alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as theSecondary antibody.

Bioworld Molecular Weight: 55 kDa

Specificity: RARα polyclonal antibody detects endogenous levels of RARα protein.

Alternative Name: RAR-alpha, RARA, NR1B1, NR1B1, Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein, Nuclear receptor subfamily 1 group B member 1, Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form, RAR alpha, RAR, RAR-alpha, rara, RARA, RARalpha, RARalpha1, Retinoic acid nuclear receptor alpha variant 1, Retinoic acid nuclear receptor alpha variant 2, Retinoic acid receptor alpha, Retinoic acid receptor alpha polypeptide,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified