ITI-H2 Polyclonal Antibody

Catalogue Numbers: BS5768-50, BS5768-100

Sizes: 50µl, 100µl

Alternative Name: Inter-alpha-trypsin inhibitor heavy chain H2; ITI heavy chain H2; ITI-HC2; Inter-alpha-inhibitor heavy chain 2; nter-alpha-trypsin inhibitor complex component II; Serum-derived hyaluronan-associated protein; SHAP; ITIH2; IGHEP2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P19823

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The inter-α trypsin inhibitor (ITI) family is a group of structurally related plasma serine protease inhibitors synthesized in the liver and built up from different combinations of three highly homologous heavy chains (ITI-HI, ITI-H2 and ITI-H3) and one light chain (bikunin). A fourth member of the ITI family, ITI-H4 (also known as I α IH4P) harbours a Pro-rich region (PRR) in its c-terminus. ITI (also known as IaI) is a 220 kDa glycoprotein composed of three polypeptides linked by chondroitin sulphate: two heavy chains, ITI-H1 (65 kDa) and ITI-H2 (72 kDa), and bikunin (approx. 30 kDa). Bikunin confers the protease-inhibitor function of ITI. The heavy chains of the ITI family, designated as SHAPs (for serum-derived hyaluronan-associated proteins), bind
covalently to hyaluronic acid (HA), resulting in pericellular matrix stabilization. ITI-H1 contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ITI-H2 polyclonal antibody detects endogenous levels of mature ITI-H2 protein.

Bioworld Molecular Weight: ~ 72, 106 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of ITI-H2 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 712-757 of Human ITI-H2.

Conjugate: Unconjugated

Modification: Unmodified