ILK Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90713-50, BS90713-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13418 Human;O55222 Mouse;Q99J82 Rat

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC

All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200

Background: Integrins are heterodimers composed of non-covalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind to ligands that are components of the extracellular matrix. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. ILK (integrin-linked kinase) was identified as a serine/threonine kinase that phosphorylates b1 and b3 integrins. ILK expression has been shown to be reduced in response to Fibronectin, a known integrin ligand. Overexpression of ILK was shown to upregulate the Fibronectin matrix assembly in epithelial cells, indicating a potential role for ILK in cell growth, cell survival and tumorigenesis.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: ILK polyclonal antibody detects endogenous levels of ILK protein.

Bioworld Molecular Weight: 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Integrin linked ILK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat lung tissue lysate, ICC staining Integrin linked ILK in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Integrin linked ILK polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Alternative Name: ILK1, ILK2, ILK, 59 kDa serine/threonine protein kinase, 59 kDa serine/threonine-protein kinase, DKFZp686F1765, Epididymis secretory protein Li 28, HEL S 28, ILK 1, ILK 2, ILK, ILK-1, ILK-2, ILK, ILK1, ILK2, Integrin linked kinase 2, Integrin linked Kinase, Integrin linked protein kinase, Integrin-linked protein kinase, p59, p59ILK antibody

Immunogen: Recombinant protein within Human Integrin linked ILK aa 1-290.

Conjugate: Unconjugated

Modification: Unmodified