Septin 1 (D205) Polyclonal Antibody

Catalogue Numbers: BS2111-50, BS2111-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8WYJ6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The septins are a family of GTPase enzymes, some of which are required for cytokinesis and others of which are associated with exocytosis. Members of the septin family can form heteropolymer complexes and also play a role in the organization of new growth in organisms. The transcriptional regulation of all septins is complex, resulting in alternatively spliced variants. At least three septins (Septin 1, 2 and 4) are associated with a Tau-based paired helical filament core and may contribute to the formation of neurofibrillary tangle as integral constituents of paired helical filaments. Septin 3 (G-Septin), a GTP-binding protein, is highly expressed in brain and is regulated by protein kinase G in neurons. The human SEPT4 (H5/PNUTL2/CDCrREL-2) gene encodes ARTS (for apoptosis-related protein in the TGFβ signaling pathway), which is expressed in many cells and acts to enhance cell death induced by TGFβ or, to a lesser extent, by other apoptotic agents.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Septin 1 (D205) polyclonal antibody detects endogenous levels of Septin 1 protein.

Extra Notes: Western blot (WB) analysis of Septin 1 (D205) pAb at 1:1000 dilution
Lane1:K562 whole cell lysate(40ug)
Lane2:SKOVCAR3 whole cell lysate(40ug)
Lane3:PC12 whole cell lysate(40ug)
Lane4:3T3-L1 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Septin-1; LARP; Peanut-like protein 3; Serologically defined breast cancer antigen NY-BR-24; SEPT1; DIFF6; PNUTL3

Immunogen: Synthetic peptide, corresponding to amino acids 169-224 of Human Septin 1.

Conjugate: Unconjugated

Modification: Unmodified