GAPDH Monoclonal Antibody

Catalogue Numbers: MB65930-50, MB65930-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P04406

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB (1/3000 - 1/10000), IHC (1/100 - 1/200)

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GAPDH protein.

Extra Notes: Western blot analysis of GAPDH expression in Hela (A), mouse brain (B), rat brain (C), rabbit muscle (D), sheep muscle (E) whole cell lysates.

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: GAPD; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human GAPDH. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified