GADD 45γ (S123) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS9140-50, BS9140-100

Sizes: 50µl, 100µl

Swiss-Prot: O95257

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

Background: Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and p21. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Thus, it has been suggested that GADD 45 may serve as a link between the p53-dependent cell cycle checkpoint and DNA repair. GADD 45-like proteins, GADD 45β and GADD 45γ, have been shown to be induced by environmental stresses. GADD 45β and GADD 45γ are thought to induce p38/JNK activation via MEKK4 activation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GADD 45γ (S123) polyclonal antibody detects endogenous levels of GADD 45γ protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of GADD 45γ (S123) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 17 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: CR6; Cytokine responsive protein CR6; Cytokine-responsive protein CR6; DDIT-2; DDIT2; DNA damage-inducible transcript 2 protein; GA45G; Gadd45g; Growth arrest and DNA damage inducible gamma; Growth arrest and DNA damage-inducible protein GADD45 gamma; GRP17;

Immunogen: Synthetic peptide, corresponding to amino acids 101-150 of Human GADD 45γ.

Conjugate: Unconjugated

Modification: Unmodified