TPH1 (K54) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1698-50, BS1698-100

Sizes: 50µl, 100µl

Swiss-Prot: P17752

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF

Background: Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TPH1 (K54) polyclonal antibody detects endogenous levels of TPH1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TPH1 (K54) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: A549 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of TPH1 (K54) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 51 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH; TPRH; TRPH

Immunogen: Synthetic peptide, corresponding to amino acids 20-70 of Human TPH1.

Conjugate: Unconjugated

Modification: Unmodified