Recombinant Human Troponin C/TNNC1 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033520-10, PKSH033520-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Troponin C; TNNC1

Target Symptom: CMD1Z; CMH13; TN-C; TNC; TNNC

Research Area: Signal Transduction; Cardiovascular;

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-His

UNIProt ID: P63316

Accession: P63316

Background: Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments.Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2(encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Sequence: Met1-Glu161

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 100mM NaCl, 1mM DTT, 10% glycerol, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 19.8 kDa

Observed MW: 17-20 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only