Recombinant Human NMNAT1/NMNAT Protein (His Tag)

Size: 50μg

Catalogue Numbers: PKSH031118-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: NMNAT1; NMNAT

Target Synonym: LCA9; NMNAT; PNAT1

UNIProt ID: Q9HAN9

Research Areas: Signal Transduction; Neuroscience; Cancer; epigenetics and nuclear signal; metabolism;

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Fusion Tag: C-His

Accession: Q9HAN9

Background: NMNAT, also known as NMNAT1, is a member of the Nicotinamide-nucleotide adenylyltransferases. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. NMNAT appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT enzyme is vital for NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD.

Sequence: Met 1-Thr 279

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 33.3 kDa

Observed MW: 34 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only