CD169 Polyclonal Antibody Catalogue Numbers: NCP0291P-50, NCP0291P-100 Sizes: 50µl, 100µl Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. Swiss-Prot: Q9BZZ2 Host: Rabbit Reactivity: Human, Rat, Mouse Applications: WB All Applications: WB: 1:1000~1:2000 Background: Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1,709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: CD169 polyclonal antibody detects endogenous levels of CD169 protein. Extra Notes: Western blot (WB) analysis of CD169 polyclonal antibody at 1:1000 dilution Lane1:RAW264.7 whole cell lysate(30ug) Lane2:Myla2059 whole cell lysate(30ug) Lane3:The Spleen tissue lysate of Rat(30ug) Bioworld Molecular Weight: ~ 200 kDa Note: For research use only, not for use in diagnostic procedure. Alternative Name: Sialoadhesin; Sialic acid-binding Ig-like lectin 1; Siglec-1; CD169; SIGLEC1; SN Immunogen: Recombinant protein of human CD169. Conjugate: Unconjugated Modification: Unmodified