ALKBH3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7871-50, BS7871-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96Q83

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:2000

Background: ALKBH3 (alkB, alkylation repair homolog 3), also known as ABH3, PCA1 (prostate cancer antigen 1), DEPC1 or DEPC-1, is a 286 amino acid member of the AlkB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. ALKBH3 is one of many homologs of the Escherichia coli protein, AlkB. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus. It associates with iron and 2-oxoglutarate, coupling the oxidation of substrates to the conversion of 2-oxoglutarate into succinate and CO2. Via oxidative demethylation, ALKBH3 repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA. Its activity is stimulated by ascorbate. Two isoforms exist for ALKBH3 due to alternative splicing of the gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ALKBH3 polyclonal antibody detects endogenous levels of ALKBH3 protein.

Bioworld Molecular Weight: ~ 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of ALKBH3 polyclonal antibody

Alternative Name: ABH3;
AlkB homolog 3;
alkB, alkylation repair homolog 3 (E. coli);
ALKB3_HUMAN;
ALKBH3;
Alkylated DNA repair protein alkB homolog 3;
alkylation repair homolog 3;
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3;
DEPC 1;
DEPC-1;
DEPC1;
EC 1.14.11.;
FLJ43614;
mABH3;
MGC118790;
MGC118792;
MGC118793;
MGC134125;
PCA1;
Prostate cancer antigen 1;
prostate cancer antigen-1;
RP23-375N21.4;

Immunogen: Recombinant full length Human ALKBH3.

Conjugate: Unconjugated

Modification: Unmodified