HSPB2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65896-50, BS65896-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: Q16082

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. HSPB2 (heat shock 27 kDa protein 2), also known as HSP 27 or MKBP, is a 182 amino acid protein that belongs to the heat shock protein family and is expressed preferentially in heart and skeletal muscle. Localized to mitochondria, HSPB2 functions as an ATP-dependent chaperone protein that plays a role in the refolding of denatured proteins and may also interact with the Actin cytoskeleton and prevent apoptotic cell death. HSPB2 is abundantly expressed in several cancer cell lines, suggesting that HSPB2 may be an important factor in tumor transformation and metastasis.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: HSPB2 Polyclonal Antibody detects endogenous levels of HSPB2 protein.

Bioworld Molecular Weight: 20kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes;Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSPB2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Alternative Name: DMPK-binding protein; Heat shock 27kD protein 2; Heat shock 27kDa protein 2; Heat shock protein beta-2; Heat-shock protein beta-2; Hs.78846; HSP27; HspB2; HSPB2_HUMAN; LOH11CR1K; MGC133245; MKBP.

Immunogen: KLH conjugated synthetic peptide derived from human HSPB2:101-182/182

Conjugate: Unconjugated

Modification: Unmodified