NRL Polyclonal Antibody

Catalogue Numbers: BS5829-50, BS5829-100

Sizes: 50µl, 100µl

Alternative Name: Neural retina-specific leucine zipper protein; D14S46E

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P54845

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Nrl (neural retina leucine zipper) is a member of the Maf family of transcription factors, which characteristically contain a highly conserved basic leucine zipper (bZIP)-DNA binding motif. Both Nrl and c-Maf preferentially bind to T-MARE sites and are implicated in a wide variety of developmental and physiologic roles. The Maf-Nrl subfamily regulates the expression of cell type-specific genes in tissues of the hematopoietic system, cerebellum and developing hindbrain. Maf and Nrl proteins bind an extended AP-1-like sequence and can form heterodimers with Fos and Jun transcription factors. In retinal cells and photoreceptor cells, Nrl promotes the expression of rhodopsin through binding to the Nrl response element present in the rhodopsin promoter. Nrl is expressed throughout the developing central and peripheral nervous system during neuronal differentiation, and its expression is restricted to neocortex, brainstem and retinal neurons during adulthood.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NRL polyclonal antibody detects endogenous levels of NRL protein.

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NRL polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 42-87 of Human NRL.

Conjugate: Unconjugated

Modification: Unmodified