PDI Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91043-50, BS91043-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07237(Human) Q13087(Human) D3Z6P0(Mouse) Unigene:65154(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100

Background: Oxidoreductase-protein disulfide isomerase (PDI) is a homodimer consisting of subunits that catalyzes thiol-disulfide exchange, mediates folding of newly synthesized proteins and functions as a molecular chaperone. PDI localizes to the lumen of the endoplasmic reticulum (ER), where in conjunction with folding-helper proteins, such as immunoglobulin heavy chain binding protein (BiP), mediates tertiary and quaternary protein-processing. Cell surface PDI induces sulfhydryl-mediated conformational changes in integrin-mediated adhesion receptor-ligand interactions, thereby regulating integrin responses and cell adhesion. Additionally, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PDI polyclonal antibody detects endogenous levels of PDI protein.

Bioworld Molecular Weight: 58 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PDI on different lysates using anti-PDI antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: Human liver, Flow cytometric analysis of Hela cells with PDI antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Alternative Name: PDIp, Pdia2, Pancreas specific protein disulfide isomerase, Pancreas-specific protein disulfide isomerase, Pancreatic protein disulfide isomerase, PDA2, PDI, PDIA2, PDIA2, PDIp, PDIR, Protein disulfide isomerase A2, Protein disulfide isomerase, Protein disulfide isomerase associated 2, Protein disulfide isomerase family A member 2, Protein disulfide isomerase pancreatic, Protein disulfide-isomerase A2, Rho GDP dissociation inhibitor gamma,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified