HSP27 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90668-50, BS90668-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04792

Host: Rabbit

Reactivity: Human, Mouse, rat

Applications: WB, ICC IHC

All Applications: WB:1:500
ICC:1:100
IHC:1:200

Background: Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, α-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. Hsp27 appears in many cell types, especially all types of muscle cells. It is located mainly in the cytosol, but also in the perinuclear region, endoplasmatic reticulum, and nucleus. It is overexpressed during different stages of cell differentiation and development. This suggests an essential role for Hsp27 in the differentiation of tissues. Probably Hsp27 – among other chaperones – is involved in the process of cell differentiation. Changes of Hsp27 levels were observed in Ehrlich ascite cells, embryonic stem cells, normal B-cells, B-lymphoma cells, osteoblasts, keratinocytes, neurons etc.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: HSP27 polyclonal antibody detects endogenous levels of HSP27 protein.

Bioworld Molecular Weight: 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Hsp27 on different cell lysates using anti- Hsp27 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: 293
Lane 4: Human kidney
Lane 5: Mouse kidney
Lane 6: Mouse heart, ICC staining Hsp27 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HspB1, HSP 27, SRP27, Heat shock 27kDa protein, 28 kDa heat shock protein, CMT2F, DKFZp586P1322, epididymis secretory protein Li 102, Estrogen regulated 24 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat shock protein beta-1, heat shock protein family B (small) member 1, HEL-S-102, HMN2B, HS.76067, Hsp 25, HSP 27, Hsp 28, Hsp B1, Hsp25, HSP27, Hsp28, HspB1, HSPB1, SRP27, Stress responsive protein 27, Stress-responsive protein 27,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified