NFIB Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS76230-50, BS76230-100

Swiss-Prot: O00712

Host: Rabbit

NFIB Polyclonal Antibody-BS76230

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Rat

Applications: WB, IHC

All Applications: WB,1:200 - 1:2000 | IHC,1:50 - 1:200

Background: Transcriptional activator of GFAP, essential for proper brain development. Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 47kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using NFIB antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s., Immunohistochemistry of paraffin-embedded rat lung using NFIB Rabbit pAb at dilution of 1:250.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: NFIB; CTF; HMGIC/NFIB; NF-I/B; NF1-B; NFI-B; NFI-RED; NFIB2; NFIB3

Immunogen: A synthetic peptide of human NFIB(NP_005587.2).

Modification: Unmodified

Conjugate: Unconjugated