DDX3Y Polyclonal Antibody

Catalogue Numbers: BS61139-50, BS61139-100

Sizes: 50µl, 100µl

Alternative Name: ATP-dependent RNA helicase DDX3Y (EC:3.6.4.13); DEAD box protein 3, Y-chromosomal; DDX3Y; DBY

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15523

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3 (DEAD box polypeptide 3) is involved in RNA metabolism. Two DDX3 paralogs are found in humans; DDX3X is encoded by a gene found on the X chromosome while DDX3Y is encoded by a gene on the Y chromosome. DDX3Y is exclusively expressed in testis and is required for normal spermatogenesis. DDX3X is ubiquitously expressed and predominantly localizes to the nuclear speckles, participating in RNA splicing, transcription, translation initiation, mRNA transport and cell cycle regulation. DDX3X also partakes in HIV-1 replication and hepatitis C viral infections.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DDX3Y polyclonal antibody detects endogenous levels of DDX3Y protein.

Bioworld Molecular Weight: ~ 73 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DDX3Y polyclonal antibody at 1:500 dilution
Line1:THP-1 whole cell lysate
Line2:PC12 whole cell lysate
Line3:sp20 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human DDX3Y.

Conjugate: Unconjugated

Modification: Unmodified