4E BP1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10871-50, MB10871-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q13541

Host: Rabbit

Reactivity: Human, Mouse, Rat, Hamster

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 13 kDa; Observed MW: 15-20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of eIF4EBP1 in mouse heart lysates using 4E BP1 antibody., Western blot analysis of eIF4EBP1 in Jurkat, C6, CHO-K1 lysates using eIF4EBP1 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using eIF4EBP1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

Immunogen: A synthetic peptide of human eIF4EBP1

Conjugate: Unconjugated

Modification: Unmodified