CD282 Monoclonal Antibody

Catalogue Numbers: MB65768-50, MB65768-100

Sizes: 50µl, 100µl

Product: Mouse IgG2b. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: O60603

Host: Mouse

Reactivity: Human

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.
TLR2 is expressed on the surface of monocytes and macrophages and can heterodimerize with TLR1 or TLR6, enabling responses to a variety of pathogen-associated molecular patterns including lipopetides and peptidoglycan.

Purification and Purity: The monoclonal antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes human CD282

Extra Notes: Flow cytometric analysis of human peripheral blood monocytes using Anti-CD282 Antibody, followed by anti-mouse IgG PE.

Bioworld Molecular Weight: ~ 89 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: TIL4; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; CD antigen CD282

Immunogen: Native purified human CD282.

Conjugate: Unconjugated

Modification: Unmodified