PGK1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91064-50, BS91064-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P00558(Human) P09411(Mouse) P16617(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IP, , FC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
FC:1:50-1:100

Background: Phosphoglycerate kinases 1/2 (PGK1/2, ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) are somatically expressed, glycolytic enzymes that catalyze the transfer of a phosphoryl group from the acyl phosphate of 1,3-bisphosphoglycerate to ADP, thereby forming ATP and 3-phosphoglycerate. The human PGK gene is interrupted by 10 introns and spans 23 kilobases, and is X chromosome-linked at position Xq21.1, a region implicated in prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. In addition to influencing glycolysis, the PGK1 is secreted by tumor cells and contributes to proliferative angiogenic processes as a disulfide reductase. PGK1 mediated reduction of disulphide bonds in the serine proteinase plasmin initiates the release of the tumor blood vessel inhibitor angiostatin, an event that is critical for blood vessel formation or angiogenesis in tumor expansion and metastasis.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PGK1 polyclonal antibody detects endogenous levels of PGK1 protein.

Bioworld Molecular Weight: 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PGK1 on different lysates using anti-PGK1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: MCF-7, ICC staining PGK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PRP 2, Cell migration-inducing gene 10 protein, Epididymis secretory sperm binding protein Li 68p, HEL S 68p, MGC117307, MGC8947, MIG10, pgk1, PGK1, PGKA, Phosphoglycerate kinase 1, Primer recognition protein 2, PRP 2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified