Phospho-AURKA-T288/AURKB-T232/AURKC-T198 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74196-50, BS74196-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14965/Q96GD4/Q9UQB9

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-AURKA-T288/AURKB-T232/AURKC-T198 antibody at 1:1000 dilution. Hela cells were treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 35KkDa/48kDa

Specificity: Phosphorylated

Alternative Name: AURKA/AURKB/AURKC

Immunogen: A synthetic phosphorylated peptide around T288 of human AURKA/AURKB/AURKCAURKA(NP_003591.2).

Conjugate: Unconjugated

Modification: Phosphorylated